Popović, Lukša; Rossner, Moritz J.; Wehr, Michael C. (2024): Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay. STAR Protocols, 5 (2): 102987. ISSN 26661667
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Abstract
The ERBBprofiler assay measures compound effects on ERBB family receptors and key downstream signaling pathways that are implicated in cancer or other complex diseases. Here, we present a protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay. We describe steps for in-solution transfection, cell treatment, combined processing of samples, amplification and indexing of PCRs, sequencing, and data analysis. This approach allows for the simultaneous assessment of drug effects and cell-type-dependent effects.
| Doc-Type: | Article (LMU Hospital) |
|---|---|
| Organisational unit (Faculties): | 07 Medicine > Medical Center of the University of Munich > Clinic and Polyclinic for Psychiatry and Psychotherapy |
| DFG subject classification of scientific disciplines: | Life sciences |
| Date Deposited: | 04. Sep 2024 09:24 |
| Last Modified: | 11. Nov 2024 14:29 |
| URI: | https://oa-fund.ub.uni-muenchen.de/id/eprint/1442 |
| DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 491502892 |
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