Vincek, Anna; Wolf, Anja; Thomas, Astrid; Ebel, Frank ORCID: 0000-0002-1027-1440; Schruefer, Sebastian ORCID: 0000-0002-8542-1300 (2024): The N-terminus of the Aspergillus fumigatus group III hybrid histidine kinase TcsC is essential for its physiological activity and targets the protein to the nucleus. mBio. ISSN 2150-7511
vincek-et-al-2024-the-n-terminus-of-the-aspergillus-fumigatus-group-iii-hybrid-histidine-kinase-tcsc-is-essential-for.pdf
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Abstract
Group III hybrid histidine kinases are fungal-specific proteins and part of the multistep phosphorelay, representing the initial part of the high osmolarity glycerol (HOG) pathway. TcsC, the corresponding kinase in Aspergillus fumigatus, was expected to be a cytosolic protein but is targeted to the nucleus. Activation of TcsC by the antifungal fludioxonil has lethal consequences for the fungus. The agent triggers a fast and TcsC-dependent activation of SakA and later on a redistribution of TcsC to the cytoplasm. High osmolarity also activates TcsC, which then exits the nucleus or concentrates in spot-like, intra-nuclear structures. The sequence corresponding to the N-terminal 208 amino acids of TcsC lacks detectable domains. Its loss renders TcsC cytosolic and non-responsive to hyperosmotic stress, but it has no impact on the antifungal activity of fludioxonil. A point mutation in one of the three putative nuclear localization sequences, which are present in the N-terminus, prevents the nuclear localization of TcsC, but not its ability to respond to hyperosmotic stress. Hence, this striking intracellular localization is no prerequisite for the role of TcsC in the adaptive response to hyperosmotic stress, instead, TcsC proteins that are present in the nuclei seem to modulate the cell wall composition of hyphae, which takes place in the absence of stress. The results of the present study underline that the spatiotemporal dynamics of the individual components of the multistep phosphorelay is a crucial feature of this unique signaling hub.
Doc-Type: | Article (LMU) |
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Organisational unit (Faculties): | 08 Veterinary Medicine > Department of Veterinary Medicine > Lehrstuhl für Bakteriologie und Mykologie |
DFG subject classification of scientific disciplines: | Life sciences |
Date Deposited: | 18. Jul 2024 08:54 |
Last Modified: | 18. Jul 2024 08:54 |
URI: | https://oa-fund.ub.uni-muenchen.de/id/eprint/1306 |
DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 427720308 |
DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 491502892 |