Two domains of Tim50 coordinate translocation of proteins across the two mitochondrial membranes

Genge, Marcel G; Roy Chowdhury, Shalini; Dohnálek, Vít; Yunoki, Kaori; Hirashima, Takashi; Endo, Toshiya; Doležal, Pavel; Mokranjac, Dejana (2023): Two domains of Tim50 coordinate translocation of proteins across the two mitochondrial membranes. Life Science Alliance, 6 (12): e202302122. ISSN 2575-1077

Abstract

Hundreds of mitochondrial proteins with N-terminal presequences are translocated across the outer and inner mitochondrial membranes via the TOM and TIM23 complexes, respectively. How translocation of proteins across two mitochondrial membranes is coordinated is largely unknown. Here, we show that the two domains of Tim50 in the intermembrane space, named core and PBD, both have essential roles in this process. Building upon the surprising observation that the two domains of Tim50 can complement each other in trans, we establish that the core domain contains the main presequence-binding site and serves as the main recruitment point to the TIM23 complex. On the other hand, the PBD plays, directly or indirectly, a critical role in cooperation of the TOM and TIM23 complexes and supports the receptor function of Tim50. Thus, the two domains of Tim50 both have essential but distinct roles and together coordinate translocation of proteins across two mitochondrial membranes.

• Doc-Type: Article (LMU)
• Organisational unit (Faculties): 19 Biology > Department Biology II
• DFG subject classification of scientific disciplines: Life sciences
• DFG: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 450889947
• DFG: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 491502892