Fan, Yanxin; Mizoguchi, Yoko; Tatematsu, Megumi; Linder, Monika I.; Frenz, Stephanie; Choi, Jongsu; Klein, Christoph (2023): Analyzing mitochondrial respiration of human induced pluripotent stem cell-derived myeloid progenitors using Seahorse technology. STAR Protocols, 4 (1). p. 102073. ISSN 26661667
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1-s2.0-S266616672300031X-main.pdf
Abstract
Mitochondrial metabolism is critical in hematopoietic stem cell maintenance and differentiation. Here, we present a step-by-step protocol to efficiently differentiate human induced pluripotent stem cells into myeloid progenitors by a robust feeder- and serum-free system. Furthermore, we provide a protocol to subsequently assess mitochondrial function in iPSC-derived myeloid progenitors. We comprehensively describe a protocol to analyze and to quantify key parameters of mitochondrial respiration of iPSC-derived myeloid progenitors by the Seahorse XFe96 Analyzer. Additionally, our protocol includes extensive troubleshooting suggestions.
| Doc-Type: | Article (LMU Hospital) |
|---|---|
| Organisational unit (Faculties): | 07 Medicine > Medical Center of the University of Munich > Paediatric Surgery Clinic and Polyclinic in the Dr. von Hauner Children's Hospital |
| DFG subject classification of scientific disciplines: | Life sciences |
| Date Deposited: | 16. May 2023 13:15 |
| Last Modified: | 07. Dec 2023 12:17 |
| URI: | https://oa-fund.ub.uni-muenchen.de/id/eprint/644 |
| DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 165054336 |
| DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 491502892 |
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