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Janjic, Aleksandar; Wange, Lucas E.; Bagnoli, Johannes W.; Geuder, Johanna; Nguyen, Phong; Richter, Daniel; Vieth, Beate; Vick, Binje; Jeremias, Irmela; Ziegenhain, Christoph; Hellmann, Ines; Enard, Wolfgang ORCID: 0000-0002-4056-0550 (2022): Prime-seq, efficient and powerful bulk RNA sequencing. Genome Biology, 23: 88. ISSN 1474-760X

[thumbnail of s13059-022-02660-8.pdf] Published Article

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Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.

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