Protocol for in vivo analysis of muscle function in porcine models for muscular dystrophies

Hristov, Hristiyan; Blasi, Michaela; Neves Barbosa, Igor; Kemter, Elisabeth; Kurome, Mayuko; Kessler, Barbara; Zakhartchenko, Valeri; Klymiuk, Nikolai; Stirm, Michael; Jaudas, Florian; Wolf, Eckhard (2026): Protocol for in vivo analysis of muscle function in porcine models for muscular dystrophies. STAR Protocols, 7 (1): 104369. ISSN 26661667

Abstract

Porcine models carrying DMD mutations recapitulate Duchenne and Becker muscular dystrophy (DMD and BMD), inherited disorders leading to progressive muscle weakness, with DMD presenting a far more severe and rapidly progressive phenotype. Accurate in vivo muscle function assessment is essential for monitoring disease progression and as an efficacy readout for therapeutic intervention. We present a protocol to evaluate muscle strength, dynamics, and fatigue in dystrophic pigs, including design setup, anesthesia, measurement, and data analysis.

• Dokumententyp: Artikel (LMU)
• Organisationseinheit (Fakultäten): 08 Tiermedizin > Veterinärwissenschaftliches Department > Lehrstuhl für Molekulare Tierzucht und Biotechnologie
• DFG-Fachsystematik der Wissenschaftsbereiche: Lebenswissenschaften
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892