Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

Eckstein, Yasmin; Kessler, Barbara; Hinrichs, Arne; Novak, Istvan; von Thaden, Anne; Lorenzen, Timo; Rathkolb, Birgit; Scholz, Armin; Blutke, Andreas; Koopmans, Sietse-Jan; de Angelis, Martin Hrabĕ; Christoffersen, Berit Østergaard; Wolf, Eckhard; Renner, Simone (2025): Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. STAR Protocols, 6 (2): 103774. ISSN 26661667

Abstract

The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis.

• Doc-Type: Article (LMU)
• Organisational unit (Faculties): 08 Veterinary Medicine > Department of Veterinary Medicine > Lehrstuhl für Molekulare Tierzucht und Biotechnologie
• DFG subject classification of scientific disciplines: Natural sciences
• DFG: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 536691227
• DFG: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 491502892