Keck, Maximilian; Hermann, Christian; Lützel, Kyra; Gudermann, Thomas; Konrad, David B.; Mederos y Schnitzler, Michael; Storch, Ursula (2024): Photoswitchable TRPC6 channel activators evoke distinct channel kinetics reflecting different gating behaviors. iScience, 27 (10): 111008. ISSN 2589-0042
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Abstract
The non-selective transient receptor potential canonical 6 (TRPC6) cation channels have several physiological and pathophysiological effects. They are activated by the lipid second messenger diacylglycerol (DAG) and by non-lipidic compounds such as GSK 1702934A (GSK). Advances in photopharmacology led to the development of photoswitchable activators such as PhoDAG, OptoDArG, and OptoBI-1 that can be switched ON and OFF with the spatiotemporal precision of light. We aimed to elucidate whether these photopharmaceuticals allow for a reliable determination of the ion channel current kinetics. We performed electrophysiological whole-cell measurements in the overexpression system and analyzed TRPC6 currents induced by photoswitching. We observed distinct activation, deactivation and inactivation current kinetics suggesting that each photoswitchable activator elicits a distinct active channel state. Notably, the current kinetics strongly depended on the intensity of the light source. Altogether, photopharmaceuticals are advantageous for an extended biophysical characterization of whole-cell currents and provide insight into their gating mechanism.
| Doc-Type: | Article (LMU) |
|---|---|
| Organisational unit (Faculties): | 07 Medicine > Walther Straub Institute of Pharmacology and toxicology |
| DFG subject classification of scientific disciplines: | Life sciences |
| Date Deposited: | 02. May 2025 09:37 |
| Last Modified: | 02. May 2025 09:37 |
| URI: | https://oa-fund.ub.uni-muenchen.de/id/eprint/1704 |
| DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 239283807 |
| DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 491502892 |
| DFG: | Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 503157935 |
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