Extracellular vesicle-mediated trafficking of molecular cues during human brain development

Forero, Andrea; Pipicelli, Fabrizia; Moser, Sylvain; Baumann, Natalia; Grätz, Christian; Gonzalez Pisfil, Mariano; Pfaffl, Michael W.; Pütz, Benno; Kielkowski, Pavel; Cernilogar, Filippo M.; Maccarrone, Giuseppina; Di Giaimo, Rossella; Cappello, Silvia (2024): Extracellular vesicle-mediated trafficking of molecular cues during human brain development. Cell Reports, 43 (10): 114755. ISSN 22111247

Abstract

Cellular crosstalk is an essential process influenced by numerous factors, including secreted vesicles that transfer nucleic acids, lipids, and proteins between cells. Extracellular vesicles (EVs) have been the center of many studies focusing on neurodegenerative disorders, but whether EVs display cell-type-specific features for cellular crosstalk during neurodevelopment is unknown. Here, using human-induced pluripotent stem cell-derived cerebral organoids, neural progenitors, neurons, and astrocytes, we identify heterogeneity in EV protein content and dynamics in a cell-type-specific and time-dependent manner. Our results support the trafficking of key molecules via EVs in neurodevelopment, such as the transcription factor YAP1, and their localization to differing cell compartments depending on the EV recipient cell type. This study sheds new light on the biology of EVs during human brain development.

• Doc-Type: Article (LMU)
• Organisational unit (Faculties): 07 Medicine > Institute of Physiology
• DFG subject classification of scientific disciplines: Life sciences
• DFG: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 391970916
• DFG: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - 491502892